Composite

Part:BBa_K3417001:Design

Designed by: Qitong Luan   Group: iGEM20_Gaston_Day_School   (2020-06-25)


ArgK Production Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 909


Design Notes

We use strong constitutive promoter and RBS to maximize the production. We removed the restriction sites for EcoRI to optimize the sequence.


Source

Pseudomonas syringae pv. phaseolicola strain NPS3121

References